Primer

Part:BBa_K4496011

Designed by: Larissa de Souza Kawanisi, Awana da Silva Lima, Paula Macedo Cunha, Gabriel Kimijima Itaziki   Group: iGEM22_USP-EEL-Brazil   (2022-09-20)


BJ46a gene amplification primer for pPIC9K (REV)

This is reverse primer for amplification of BJ46a (BBa_K4496002), an inbitor of jararhagin, a highly hemorrhagic protein from the venom of the Bothrops Jararaca, optimized for Pichia Pastoris, in pPIC9k.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None